Abstract |
We tried to put the estrogen metabolite to use in tumor imaging. The antibody against estriol 3-sulfate (E3 3-S), which was one of the major metabolites of estrogen in hormone-dependent mammary carcinoma, was prepared and the tissue distribution and imaging of human breast carcinoma with anti-E3 3-S antibody (Ab) were studied in nude mice. In hormone-dependent breast carcinoma, MCF-7,-bearing nude mice, [125I] anti-E3 3-S Ab localized in tumor with the percentage injected dose/g of 9.29 +/- 3.01 (mean +/- SD). This value was significantly high compared with that in hormone-independent breast carcinoma, MDA-MB-231,-bearing nude mice. At 72 h after the administration of [125I]anti-E3 3-S Ab to MCF-7 bearing mice, tumor/blood, tumor/liver and tumor/muscle ratios were 0.49, 5.02 and 6.83, respectively. These ratios were supposed to be enough for imaging. In radioimmunoscintigraphy, a MCF-7 tumor was clearly visualized at 120 or 168 h post-injection of [131I]anti-E3 3-S Ab.
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Authors | N Shimura, S Kojima, A Kubodera, T Tsuruo |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 22
Issue 5
Pg. 547-53
(Jul 1995)
ISSN: 0969-8051 [Print] United States |
PMID | 7581162
(Publication Type: Journal Article)
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Chemical References |
- Iodine Radioisotopes
- estriol 3-sulfate
- Estriol
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Topics |
- Animals
- Diagnosis, Differential
- Estriol
(analogs & derivatives, pharmacokinetics)
- Female
- Humans
- Iodine Radioisotopes
- Isotope Labeling
- Mammary Neoplasms, Experimental
(diagnosis, diagnostic imaging, physiopathology)
- Mice
- Mice, Nude
- Neoplasms, Hormone-Dependent
(diagnosis, diagnostic imaging, physiopathology)
- Radioimmunodetection
- Tissue Distribution
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