Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.

Abstract

Nucleotide sequences of normal mouse liver tRNAPhe and tumor-specific tRNAPhes isolated from Ehrlich ascites tumor and neuroblastoma cells were examined by post-labeling techniques. The results showed that their sequences are identical, except for changes in post-transcriptional modifications that are located in the anticodon region. Normal mouse liver tRNAPhe contained Cm32, Gm34 and YOH37. On the other hand, tumor-specific tRNAPhes were found in one of two possible configurations: 1) Cm32, Gm34 and Y*OH37 (under-modified YOH) or 2) C32, G34 and m1G37. The ratio of the two forms of tRNAPhes differed in different tumor cells; Ehrlich ascites tumor tRNAPhe had mainly Y*OH-containing tRNAPhe whereas neuroblastoma tRNAPhe has predominantly m1G-containing tRNAPhe. It was concluded that tumor-specific tRNAPhes are products of different extents of modification, rather than of new tRNA transcription.

• Authors: Y Kuchino, E Borek, D Grunberger, J F Mushinski, S Nishimura
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 10 Issue 20 Pg. 6421-32 (Oct 25 1982) ISSN: 0305-1048 [Print] England
• PMID: 6924749 (Publication Type: Journal Article)

Chemical References

• Anticodon
• RNA, Transfer, Amino Acyl
• wye
• Guanine

Topics

• Animals
• Anticodon (genetics)
• Base Sequence
• Carcinoma, Ehrlich Tumor (genetics)
• Guanine (analogs & derivatives, metabolism)
• Liver (metabolism)
• Mice
• Neoplasms, Experimental (genetics)
• Neuroblastoma (genetics)
• Nucleic Acid Conformation
• RNA Processing, Post-Transcriptional
• RNA, Transfer, Amino Acyl (genetics, isolation & purification)