Evaluation of di-(2-ethylhexyl)phthalate and its major metabolites in the Ames test and L5178Y mouse lymphoma mutagenicity assay.

Abstract

Di-(2-ethylhexyl)phthalate (DEHP) and its two major metabolites, mono-(2-ethylhexyl)phthalate (MEHP) and 2-ethylhexanol (EH), were tested for genetic activity in both the Salmonella/mammalian microsome mutagenicity (Ames) assay and the L5178Y TK+/- mouse lymphoma cell mutagenicity assay. All chemicals were tested in both the presence and absence of Aroclor-induced liver microsomes prepared from male Sprague-Dawley rats. Dose levels for both assays were selected from preliminary toxicity studies for each chemical. Neither DEHP, MEHP, nor EH exhibited any significant mutagenic activity in strains TA-98, TA-100, TA-1535, TA-1537, and TA-1538 in the Ames test or when tested in the L5178Y TK+/- mouse lymphoma cell mutagenicity assay.

• Authors: P E Kirby, R F Pizzarello, T E Lawlor, S R Haworth, J R Hodgson
• Journal: Environmental mutagenesis (Environ Mutagen) Vol. 5 Issue 5 Pg. 657-63 ( 1983) ISSN: 0192-2521 [Print] United States
• PMID: 6352251 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Hexanols
• Mutagens
• Phthalic Acids
• Plasticizers
• Diethylhexyl Phthalate
• mono-(2-ethylhexyl)phthalate
• 2-ethylhexanol

Topics

• Animals
• Biotransformation
• Diethylhexyl Phthalate (analogs & derivatives, toxicity)
• Hexanols (toxicity)
• Leukemia L5178 (genetics)
• Leukemia, Experimental (genetics)
• Mice
• Microsomes, Liver (metabolism)
• Mutagenicity Tests
• Mutagens
• Mutation
• Phthalic Acids (toxicity)
• Plasticizers
• Salmonella typhimurium (drug effects)
• Structure-Activity Relationship