[Detection and characterization of the plasmids of the plague microbe which determine the synthesis of pesticin I, fraction I antigen and "mouse" toxin exotoxin].

Abstract

Plasmid DNA was isolated from Yersinia pestis strains containing pesticin I or fraction I antigen and "mouse" toxin determinants. Specificity of DNA preparations was studied by using them for transformation of plague agent strains carrying no plasmids. pPstI plasmid (molecular weight 7,0-7,8 MD) encoded pesticin I, fibrinolysin and plasmacoagulase synthesis. Fraction I antigen and "mouse" toxin production determinants were borne on pFraI/Tox plasmid (molecular weight about 50 MD). The observation that some Y. pestis cultures, having lost the ability to synthesize one of pFraI/Tox products, still retained this plasmid in their cells, is regarded as an evidence for a complicated regulation of pFraI/Tox function.

• Authors: O A Protsenko, P I Anisimov, O T Mozharov, N P Konnov, Iu A Popov
• Journal: Genetika (Genetika) Vol. 19 Issue 7 Pg. 1081-90 (Jul 1983) ISSN: 0016-6758 [Print] Russia (Federation)
• Vernacular Title: Vyiavlenie i kharakteristika plazmid chumnogo mikroba, determiniruiushchikh sintez pestitsina I, antigena fraktsiia I i ékzotoksina "myshinogo" toksina.
• PMID: 6194044 (Publication Type: Journal Article)

Chemical References

• Antigens, Bacterial
• Bacteriocins
• DNA, Bacterial
• Epitopes
• Exotoxins
• Pesticin

Topics

• Antigens, Bacterial (genetics)
• Bacteriocins (biosynthesis, genetics)
• DNA, Bacterial (genetics, isolation & purification)
• Epitopes (genetics)
• Exotoxins (biosynthesis, genetics)
• Microscopy, Electron
• Plasmids
• Yersinia pestis (genetics, immunology, metabolism)