Abstract |
Friedman, Robert M. (National Cancer Institute, Bethesda, Md.). Interferon production and protein synthesis in chick cells. J. Bacteriol. 91:1224-1229. 1966.-Overnight incubation of chick embryo fibroblasts (CEF) at 4 C before infection with live Semliki Forest virus (SFV) increased virus yields but decreased interferon production. The same findings were noted when CEF were incubated for 4 hr with p-fluorophenylalanine (FPA) before infection with live SFV or inactivated Chikungunya virus. In both systems incorporation of C(14)-leucine into protein appeared to be increased after pretreatment at 4 C or with FPA. Protein synthesis could be raised in CEF incubated in 0.5% serum after trypsinization by increasing the concentration of serum. CEF in 10% serum had higher rates of C(14)-leucine incorporation than did cells in 1.5% serum, but again the cells with the apparently high rate of incorporation produced less interferon. These findings may be related to the mechanism of cellular control over interferon production.
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Authors | R M Friedman |
Journal | Journal of bacteriology
(J Bacteriol)
Vol. 91
Issue 3
Pg. 1224-9
(Mar 1966)
ISSN: 0021-9193 [Print] United States |
PMID | 5929753
(Publication Type: Journal Article)
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Chemical References |
- Carbon Isotopes
- Phenylalanine
- Interferons
- Leucine
|
Topics |
- Animals
- Blood
- Carbon Isotopes
- Chick Embryo
(metabolism)
- Chikungunya virus
- Fibroblasts
(metabolism)
- In Vitro Techniques
- Interferons
(biosynthesis)
- Leucine
(metabolism)
- Phenylalanine
(pharmacology)
- Protein Biosynthesis
- Semliki forest virus
- Virus Cultivation
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