Monoclonal antibodies to T-2 toxin. In vitro neutralization of protein synthesis inhibition and protection of rats against lethal toxemia.

Abstract

A murine monoclonal antibody (15H6) against the trichothecene mycotoxin T-2 was capable of neutralizing the in vitro protein synthesis inhibitory effect of T-2 toxin in human B lymphoblastoid cultures. It was further shown that 15H6 given to rats (250 mg/kg) 30 min before or 15 min after a lethal dose (1 mg/kg) of T-2 toxin conferred 100% survival. A lower dose of 15H6 (125 mg/kg), given 15 min after the lethal dose of T-2 toxin, protected 25% of the rats. An increased time to death and 45% survival was seen in rats given the full dose of 15H6 antibody 60 min after lethal toxin. These data are the first demonstration of effective prophylaxis and therapy for T-2 toxemia.

• Authors: G Feuerstein, J A Powell, A T Knower, K W Hunter Jr
• Journal: The Journal of clinical investigation (J Clin Invest) Vol. 76 Issue 6 Pg. 2134-8 (Dec 1985) ISSN: 0021-9738 [Print] United States
• PMID: 4077976 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antitoxins
• Sesquiterpenes
• Trichothecenes
• T-2 Toxin

Topics

• Animals
• Antibodies, Monoclonal (therapeutic use)
• Antitoxins (therapeutic use)
• Blood Pressure (drug effects)
• Cells, Cultured
• Heart Rate (drug effects)
• Neutralization Tests
• Protein Biosynthesis
• Rats
• Sesquiterpenes (antagonists & inhibitors, immunology)
• T-2 Toxin (immunology, toxicity)
• Trichothecenes (antagonists & inhibitors, immunology)