Narirutin Attenuates Cerebral Ischemia-Reperfusion Injury by Suppressing the TXNIP/NLRP3 Pathway.

Abstract

Narirutin (Nar) is a flavonoid that is abundantly present in citrus fruits and has attracted considerable attention because of its diverse pharmacological activities and low toxicity. Here, we evaluated the preventive effects of Nar in middle cerebral artery occlusion/reperfusion (MCAO/R)-injured mice and oxygen-glucose deprivation/reperfusion (OGD/R)-injured bEnd.3 cells. Pretreatment with Nar (150 mg/kg) for 7 days effectively reduced infarct volume, improved neurological deficits, and significantly inhibited neuronal death in the hippocampus and cortex in MCAO/R-injured mice. Moreover, anti-apoptotic effects of Nar (50 µM) were observed in OGD/R-injured bEnd.3 cells. In addition, Nar pre-administration regulated blood-brain barrier function by increasing tight junction-related protein expression after MCAO/R and OGD/R injury. Nar also inhibited NOD-like receptor protein 3 (NLRP3) inflammasome activation by reducing the expression of thioredoxin-interacting protein (TXNIP) in vivo and in vitro. Taken together, these results provide new evidence for the use of Nar in the prevention and treatment of ischemic stroke.

• Authors: Li Luo, Saiying Wang, Wenna Liu, Zimei Zhang, Minggao Zhao, An Liu
• Journal: Neurochemical research (Neurochem Res) Vol. 49 Issue 3 Pg. 692-705 (Mar 2024) ISSN: 1573-6903 [Electronic] United States
• PMID: 38047987 (Publication Type: Journal Article)
• Copyright: © 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Chemical References

• NLR Family, Pyrin Domain-Containing 3 Protein
• narirutin
• NLR Proteins
• Inflammasomes
• TXNIP protein, rat
• Cell Cycle Proteins
• Disaccharides
• Flavanones

Topics

• Rats
• Mice
• Animals
• NLR Family, Pyrin Domain-Containing 3 Protein (metabolism)
• NLR Proteins
• Rats, Sprague-Dawley
• Endothelial Cells (metabolism)
• Inflammasomes (metabolism)
• Reperfusion Injury (drug therapy, prevention & control, metabolism)
• Infarction, Middle Cerebral Artery (drug therapy, metabolism)
• Brain Ischemia (drug therapy, prevention & control, metabolism)
• Cell Cycle Proteins
• Disaccharides
• Flavanones