Phage Milagro: a platform for engineering a broad host range virulent phage for Burkholderia.

Abstract

IMPORTANCE:

Burkholderia infections are a significant concern in people with CF and other immunocompromising disorders, and are difficult to treat with conventional antibiotics due to their inherent drug resistance. Bacteriophages, or bacterial viruses, are now seen as a potential alternative therapy for these infections, but most of the naturally occurring phages are temperate and have narrow host ranges, which limit their utility as therapeutics. Here we describe the temperate Burkholderia phage Milagro and our efforts to engineer this phage into a potential therapeutic by expanding the phage host range and selecting for phage mutants that are strictly virulent. This approach may be used to generate new therapeutic agents for treating intractable infections in CF patients.

• Authors: Guichun Yao, Tram Le, Abby M Korn, Hannah N Peterson, Mei Liu, Carlos F Gonzalez, Jason J Gill
• Journal: Journal of virology (J Virol) Vol. 97 Issue 11 Pg. e0085023 (Nov 30 2023) ISSN: 1098-5514 [Electronic] United States
• PMID: 37943040 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents

Topics

• Humans
• Bacteriophages (genetics)
• Burkholderia (genetics)
• Host Specificity
• Anti-Bacterial Agents