Abstract |
The disposition and toxicity of lower chlorinated PCBs (LC- PCBs) with less than five chlorine substituents have received little attention. This study characterizes the distribution and metabolomic effects of PCB 52, an LC-PCB found in indoor and outdoor air, three weeks after intraperitoneal exposure of female Sprague Dawley rats to 0, 1, 10, or 100 mg/kg BW. PCB 52 exposure did not affect overall body weight. Gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis identified PCB 52 in all tissues investigated. Hydroxylated, sulfated, and methylated PCB metabolites, identified using GC-MS/MS and nontarget liquid chromatography-high resolution mass spectrometry (Nt-LCMS), were primarily found in the serum and liver of rats exposed to 100 mg/kg BW. Metabolomic analysis revealed minor effects on L-cysteine, glycine, cytosine, sphingosine, thymine, linoleic acid, orotic acid, L-histidine, and erythrose serum levels. Thus, the metabolism of PCB 52 and its effects on the metabolome must be considered in toxicity studies.
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Authors | Amanda Bullert, Xueshu Li, Zhang Chunyun, Kendra Lee, Casey F Pulliam, Brianna S Cagle, Jonathan A Doorn, Aloysius J Klingelhutz, Larry W Robertson, Hans-Joachim Lehmler |
Journal | Environmental toxicology and pharmacology
(Environ Toxicol Pharmacol)
Vol. 102
Pg. 104245
(Sep 2023)
ISSN: 1872-7077 [Electronic] Netherlands |
PMID | 37572994
(Publication Type: Journal Article)
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Copyright | Copyright © 2023 Elsevier B.V. All rights reserved. |
Chemical References |
- 2,5,2',5'-tetrachlorobiphenyl
- Polychlorinated Biphenyls
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Topics |
- Rats
- Female
- Animals
- Polychlorinated Biphenyls
(toxicity, metabolism)
- Rats, Sprague-Dawley
- Tandem Mass Spectrometry
- Gas Chromatography-Mass Spectrometry
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