Restin protein expression in non-small cell lung cancer.

Abstract

BACKGROUND:

Restin is a member of the melanoma-associated antigen (MAGE) superfamily. Its expression has been reported to be up- or downregulated in cancer. Preclinical data suggest it is a tumor suppressor. In this study, we aimed to evaluate restin expression and prognostic value in non-small cell lung cancer (NSCLC).

METHODS:

Restin expression was analyzed by immunohistochemistry in three tissue microarrays consisting of formalin-fixed/paraffin-embedded NSCLC specimens from 113 patients, represented in triplicate. Restin staining H-score was the result of the staining intensity (0-no, 1-weak, 2-moderate, and 3-strong) multiplied by the percentage of stained tumor cells; it was defined as low if 1-100, moderate if 101-200, and strong if 201-300. Haverage-score was the average H-score in the triplicate. Restin Haverage-scores were tested for correlations with clinical and pathological characteristics and patient outcome.

RESULTS:

Restin expression was localized to the cytoplasm, with nuclear enhancement, of 112/113 (99.1%) NSCLCs. Restin Haverage-scores were 0 in 1/113 (0.88%), low in 15/113 (13.3%), moderate in 48/113 (42.5%), and strong in 49/113 (43.4%) NSCLCs. Restin Haverage-scores did not correlate with NSCLC histological subtype, disease stage, recurrence/progression-free, or overall survival.

CONCLUSION:

Restin is moderately to strongly expressed in the majority of NSCLC tumors but its expression has no prognostic value in patients with NSCLC.

• Authors: Frank Aboubakar Nana, Virginie Lamberts, Delphine Hoton, Claudia Pop Stanciu, Marylène Lecocq, François M Carlier, Fabrice Duplaquet, Lionel Pirard, Charles Pilette, Benoît Bihin, Sebahat Ocak
• Journal: Thoracic cancer (Thorac Cancer) Vol. 14 Issue 23 Pg. 2302-2309 (08 2023) ISSN: 1759-7714 [Electronic] Singapore
• PMID: 37365889 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.

Chemical References

• cytoplasmic linker protein 170
• Neoplasm Proteins
• Microtubule-Associated Proteins

Topics

• Humans
• Carcinoma, Non-Small-Cell Lung (genetics)
• Lung Neoplasms (metabolism)
• Neoplasm Proteins (metabolism)
• Microtubule-Associated Proteins (metabolism)
• Prognosis