Yeast-amoeba interaction influences murine cryptococcosis.

Abstract

The virulence of Cryptococcus spp. is modulated in the natural environment through interaction with abiotic and biotic factors, and this can occasionally have implications for the progression of cryptococcosis in mammals. Hence, we evaluated whether the prior interaction of highly virulent Cryptococcus gattii strain R265 with Acanthamoeba castellanii influenced the progression of cryptococcosis. The influence of the capsule on endocytosis was evaluated using amoeba and yeast morphometrics. Mice were intratracheally infected with yeast re-isolated from the amoeba (Interaction), yeast without prior contact with the amoeba (Non-Interaction), or sterile phosphate-buffered saline (SHAM). Morbidity signs and symptoms were monitored during the survival curve, while cytokine and fungal burden measurements and histopathological analysis were performed on the 10th day post infection. Morbidity and mortality parameters in experimental cryptococcosis were influenced by the prior interaction of yeast with amoeba, which led to phenotypic changes in the cryptococcal cells, polysaccharide secretion, and their tolerance to oxidative stress. Our results suggest that a prior yeast-amoeba interaction modulates yeast virulence, which is associated with a greater tolerance to oxidative stress related to the exo-polysaccharide content and influences the progression of cryptococcal infection.

• Authors: Jessica Helen Dos Santos Carvalho, Jeana Karen Castro Nascimento, Kassia Gabriela Vieira Silva, Sebastiao Silveira Neto, Alessandra Teixeira de Macedo, Hermeson Lima França, Larissa Dos Reis Ferreira, Rayssa de Sousa Silva, Joicy Cortez Sa, Diego Gomes Ramos, Daniela de Araújo Viana Marques, Cinthia Furst, Daniel Assis Santos, Julliana Ribeiro Alves Santos, Rodrigo Assuncao Holanda
• Journal: Microbes and infection (Microbes Infect) 2023 Sep-Oct Vol. 25 Issue 7 Pg. 105153 ISSN: 1769-714X [Electronic] France
• PMID: 37244475 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Chemical References

• Polysaccharides

Topics

• Animals
• Mice
• Saccharomyces cerevisiae
• Amoeba
• Cryptococcosis (microbiology, pathology)
• Cryptococcus gattii
• Polysaccharides
• Cryptococcus neoformans
• Mammals