Orthopedic-device-related
infection is one of the most severe complications in
orthopedic surgery. To reduce the associated morbidity and healthcare costs, new prevention and treatment modalities are continuously under development. Preclinical in vivo models serve as a control point prior to clinical implementation. This study presents a mouse model of subcutaneously implanted
titanium discs, infected with Staphylococcus aureus, to fill a gap in the early-stage testing of antimicrobial
biomaterials. Firstly, three different inocula were administered either pre-adhered to the implant or pipetted on top of it following implantation to test their ability to reliably create an
infection. Secondly, the efficacy of low-dose (25 mg/kg) and high-dose (250 mg/kg)
cefazolin administered systemically in
infection prevention was assessed. Lastly,
titanium implants were replaced by
antibiotic-loaded
bone cement (ALBC) discs to investigate the efficacy of local
antibiotics in
infection prevention. The efficacy in
infection prevention of the low-dose perioperative
antibiotic prophylaxis (PAP) depended on both the inoculum and inoculation method. Bacterial counts were significantly lower in animals receiving the high dose of PAP. ALBC discs with or without the additional PAP proved highly effective in
infection prevention and provide a suitable positive control to test other prevention strategies.