Renoprotective effect of N-acetylcystein and vitamin E in bisphenol A-induced rat nephrotoxicity; Modulators of Nrf2/ NF-κB and ROS signaling pathway.

Abstract

BACKGROUND AND AIM OF THE WORK:

Bisphenol A (BPA) is a chemical product that is widely used as a plastic precursor. It acts directly on the kidney mitochondria, causing renal dysfunction. N-acetylcysteine is effective in protecting the kidneys from chemical-induced damage. Vitamin E is an antioxidant that protects cells from the damaging effects of free radicals. The aim of this study is to further evaluate and compare NAC and vitamin E to oppose the nephrotoxicity caused by BPA.

RESEARCH DESIGN AND METHODS:

Forty-two adult male rats were divided into 7 groups:  control, BPA, NAC, vitamin E, BPA plus NAC, BPA plus vitamin E, and combined BPA, NAC and vitamin E. BPA, NAC, vitamin E were given orally at doses of 50 mg/kg, 200 mg/kg, and 1000 mg/kg respectively, for 5 weeks.

RESULTS:

NAC and vitamin E groups showed improved kidney function tests and alleviated BPA-induced oxidative stress; increased GSH and decreased MDA, NO and iNOS levels. NAC and vitamin E significantly attenuated inflammation; decreased NF-κB and increased IL-4, and Nrf2, in addition there was alleviation of renal histopathology. To some extent, vitamin E administration showed significant improvement. Moreover, combined NAC and vitamin E treatment showed more significance than either NAC or vitamin E separate groups.

CONCLUSIONS:

This study determined the substantial protective effects of NAC and/or vitamin E in BPA-induced nephrotoxicity through modulation of Nrf2 with subsequent improvement of oxidative stress and inflammation. The alleviation was more significant in combined NAC and vitamin E treatment mainly through their synergistic effect on Nrf2.

• Authors: Eman Abdelrazik, Hend Mohammed Hassan, Zienab Abdallah, Alshimaa Magdy, Eman A Farrag
• Journal: Acta bio-medica : Atenei Parmensis (Acta Biomed) Vol. 93 Issue 6 Pg. e2022301 (12 16 2022) ISSN: 2531-6745 [Electronic] Italy
• PMID: 36533744 (Publication Type: Journal Article)

Chemical References

• bisphenol A
• NF-kappa B
• NF-E2-Related Factor 2
• Reactive Oxygen Species
• Vitamin E

Topics

• Animals
• Rats
• Male
• NF-kappa B (metabolism, pharmacology, therapeutic use)
• NF-E2-Related Factor 2 (metabolism, pharmacology, therapeutic use)
• Reactive Oxygen Species (adverse effects, metabolism)
• Vitamin E (adverse effects)
• Oxidative Stress
• Signal Transduction
• Inflammation (drug therapy)