Sinomenine attenuated dextran sulfate sodium-induced inflammatory responses by promoting 14-3-3θ protein and inhibiting NF-κB signaling.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The rhizome of Chinese medical plant QingTeng (scientific name: Sinomenium acutum (Thunb.) Rehd. et Wils.) is widely used by traditional medical doctors for anti-inflammation and immunoregulatory in China and other Asian countries.

AIM OF THE STUDY:

The purpose of this study was to evaluate the effects and possible mechanisms of sinomenine resistance against DSS-induced inflammation in vitro and in vivo.

MATERIALS AND METHODS:

The UC model was induced by treating female mice with 3% DSS in vivo and human colonic epithelial cells (Hcoepic) with 0.8 mg/ml DSS in vitro. The mice and Hcoepic were then treated with sinomenine. Inflammatory factors were detected using ELISA and qRT-PCR. Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 and 14-3-3θ were analyzed by bioinformatic analysis and verified by western blotting, immunofluorescent staining or immunohistochemistry.

RESULTS:

DSS-induced Hcoepic underwent high inflammation and oxidative stress conditions, whereas sinomenine reduced the uncontrolled immune microenvironment by suppressing NF-κB signaling and targeting 14-3-3θ. Knockdown of 14-3-3θ decreased the protective effect of sinomenine against DSS-induced inflammation in vitro. Moreover, sinomenine promoted 14-3-3θ protein expression and inhibited NF-κB p65 signaling in DSS-induced mice.

CONCLUSION:

These findings suggest that 14-3-3θ plays an important role in sinomenine against DSS treatment, and sinomenine could be considered a potential drug for the treatment of UC.

• Authors: Yan Zhou, Shuai Chen, Yi Dai, Liunan Wu, Ming Jin, Jie Zhao, Yuan Li, Liming Tang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 303 Pg. 116037 (Mar 01 2023) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 36526094 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Dextran Sulfate
• Morphinans
• NF-kappa B
• sinomenine

Topics

• Animals
• Humans
• Mice
• Colitis, Ulcerative (chemically induced, drug therapy)
• Dextran Sulfate
• Disease Models, Animal
• Inflammation (chemically induced, drug therapy)
• Mice, Inbred C57BL
• Morphinans (pharmacology)
• NF-kappa B (metabolism)
• Signal Transduction