Abstract | Objectives: Vaccination using inactivated bacteria is one of the most effective ways to protect against EHEC infection. Escherichia coli O157:H7 infections are mainly influenced by Shiga toxins (Stx) and attaching/effacing factors. Among various factors, Stx2B is gaining much attention as a vaccine candidate. Formulating an inactivated bacteria with a suitable adjuvant increases vaccine efficacy and antibody production and can lead to a lasting immune response and protection against O157:H7. Materials and Methods: Results: Immunity and protection against E. coli O157:H7 were achieved by all forms of the vaccine. Inactivated E. coli O157:H7 formulated with chitosan-coated Stx2B effectively evoked humoral and mucosal immune responses. However, minimum shedding appeared with the mice groups orally immunized with formalin-inactivated bacteria sublimated with chitosan-coated Stx2B and heat-inactivated bacteria plus Stx2B in subcutaneous immunization. Conclusion: Administration of inactivated whole-cell and toxin was synergistic and increased the protection capacity with both parenteral and oral immunization routes.
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Authors | Nasim Arshadi, Seyed Latif Mousavi Gargari, Jafar Amani, Shahram Nazarian |
Journal | Iranian journal of basic medical sciences
(Iran J Basic Med Sci)
Vol. 25
Issue 9
Pg. 1069-1076
(Sep 2022)
ISSN: 2008-3866 [Print] Iran |
PMID | 36246065
(Publication Type: Journal Article)
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