PUS7 promotes the proliferation of colorectal cancer cells by directly stabilizing SIRT1 to activate the Wnt/β-catenin pathway.

Abstract

Pseudouridine synthase 7 (PUS7) may play key roles in cancer development. However, few studies have been conducted in this area. In the present study, we explored the function and potential mechanisms of PUS7 in colorectal cancer (CRC) progression. We found that PUS7 had higher expression in CRC tissues and cell lines. Clinically, high expression of PUS7 was associated with an unfavorable prognosis for CRC patients. Functionally, knockdown of PUS7 suppressed the proliferation of CRC cells in vitro and inhibited tumorigenicity in vivo. Mechanistically, RNA sequencing and coimmunoprecipitation (Co-IP) indicated that PUS7 exhibited oncogenic functions through the interaction of Sirtuin 1 (SIRT1) and activated the Wnt/β-catenin signaling pathway. Thus, our findings suggest that PUS7 promotes the proliferation of CRC cells by directly stabilizing SIRT1 to activate the Wnt/β-catenin pathway.

• Authors: Qi Zhang, Sujuan Fei, Yanchao Zhao, Shengnan Liu, Xiaoting Wu, Lili Lu, Weichang Chen
• Journal: Molecular carcinogenesis (Mol Carcinog) Vol. 62 Issue 2 Pg. 160-173 (02 2023) ISSN: 1098-2744 [Electronic] United States
• PMID: 36222184 (Publication Type: Journal Article)
• Copyright: © 2022 Wiley Periodicals LLC.

Chemical References

• beta Catenin
• SIRT1 protein, human
• Sirtuin 1
• PUS7 protein, human
• Intramolecular Transferases

Topics

• Humans
• beta Catenin (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation (genetics)
• Colorectal Neoplasms (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Sirtuin 1 (genetics, metabolism)
• Wnt Signaling Pathway (genetics)
• Intramolecular Transferases (genetics, metabolism)