Abstract |
Apocynin ( APO), a specific nicotinamide adenine dinucleotide phosphate- oxidase (NADPH- oxidase, NOX) inhibitor, has recently emerged as a bioactive phytochemical with eminent anti-inflammatory and anti-oxidant activities. To our knowledge, no research has been conducted to fabricate a mucoadhesive nanostructured delivery system of APO that targets the liver. Accordingly, chitosan (CS) surface decorated polymeric nanoparticulate delivery system (PNDS) was victoriously fabricated by double emulsion- solvent evaporation method. Herein, a randomized full 33 factorial design was employed to assess the impact of the independently processing parameters (IPPs) namely; ( poly(d,l-lactide-co-glycolide) (PLGA) amount (A)), ( polyvinyl alcohol (PVA) concentration (B)), and (CS concentration (C)), on different dependently measured attributes (DMAs). The optimal APO-loaded chitosan-coated poly(d,l-lactide-co-glycolide) nanoparticles (APO-loaded CS-coated PLGA NPs) formula (F19) would be extensively appraised through meticulous in vitro-in vivo studies. Crucially, the results revealed that oral pre-treatment with the optimal formula evoked a prodigious in vivo hepatoprotective efficacy against lipopolysaccharide (LPS)/D-(+)- galactosamine (D-GalN) induced fulminant hepatitis (FH) in BALB/c mice when compared with pure APO, uncoated F19, and plain NPs (P NPs) pretreated groups. In conclusion, APO-loaded CS-coated PLGA NPs could be considered as a promising oral mucoadhesive phytopharmaceutical PNDS to open new prospects for therapeutic intervention in inflammatory based liver diseases.
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Authors | Hend Mohamed Anter, Reham Mokhtar Aman, Ahmed Abdelaziz Shaaban, Irhan Ibrahim Abu Hashim, Mahasen Mohamed Meshali |
Journal | International journal of pharmaceutics
(Int J Pharm)
Vol. 626
Pg. 122165
(Oct 15 2022)
ISSN: 1873-3476 [Electronic] Netherlands |
PMID | 36089210
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- Acetophenones
- acetovanillone
- Antioxidants
- Chitosan
- Emulsions
- Galactosamine
- Lipopolysaccharides
- NADP
- Oxidoreductases
- Phytochemicals
- Polylactic Acid-Polyglycolic Acid Copolymer
- Polyvinyl Alcohol
- Solvents
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Topics |
- Animals
- Mice
- Acetophenones
- Antioxidants
- Chitosan
- Emulsions
- Galactosamine
- Lipopolysaccharides
- Massive Hepatic Necrosis
- Mice, Inbred BALB C
- NADP
- Nanoparticles
- Oxidoreductases
- Particle Size
- Phytochemicals
- Polylactic Acid-Polyglycolic Acid Copolymer
- Polyvinyl Alcohol
- Solvents
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