Inducible motor neuron differentiation of human induced pluripotent stem cells in vivo.

Abstract

OBJECTIVES:

Transplantation of neural progenitor cells (NPCs) derived from human-induced pluripotent stem cells (hiPSCs) is one of the promising treatment strategies for motor neuron diseases (MNDs). However, the inefficiency in committed differentiation of NPCs in vivo limits its application. Here, we tried to establish a potential therapeutic strategy for MNDs by in vivo directional differentiation of hiPSCs engineered with motor neuron (MN) specific transcription factors and Tet-On system.

MATERIALS AND METHODS:

We engineered hiPSCs with three MN-specific transcription factors and Tet-On system. The engineered cells were directly transplanted into immunodeficient mice through subcutaneous, intra-spinal cord and intracerebroventricular injections. Following doxycycline (Dox) induction, teratoma formation, and motor MN differentiation were evaluated.

RESULTS:

We generated genetically engineered hiPSCs, in which the expression of Ngn2, Isl1, and Lhx3 was controlled by a drug-inducible transgenic system. These cells showed normal pluripotency and proliferative capacity, and were able to directionally differentiate into mature motor neurons (MNs) and NPCs with high efficiency in spinal cords and cerebral lateral ventricles under the induction of Dox. The grafts showed long-term survival in the recipient mice without formation of teratoma.

CONCLUSIONS:

The induced mature MNs and NPCs were expected to replace the damaged endogenous MNs directly, and play a role of de novo stem cell stock for long-term neuron damage repair, respectively. Therefore, in vivo directional differentiation of the hiPSCs engineered with MN-specific transcription factors and Tet-On system via Dox induction could be a potential therapeutic strategy for MNDs with high efficacy and safety.

• Authors: Min Chen, Xia Wang, Chuan Li, Ting Lan, Yuhui Wei, Chengcheng Tang, Xiaoqing Zhou, Renping Zhou, Alessandro Rosa, Xi Zheng, Song Ang, Kun Zhang, Qingjian Zou, Liangxue Lai
• Journal: Cell proliferation (Cell Prolif) Vol. 55 Issue 11 Pg. e13319 (Nov 2022) ISSN: 1365-2184 [Electronic] England
• PMID: 35943218 (Publication Type: Journal Article)
• Copyright: © 2022 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd.

Chemical References

• Transcription Factors

Topics

• Humans
• Mice
• Animals
• Induced Pluripotent Stem Cells
• Motor Neurons (metabolism)
• Cell Differentiation
• Transcription Factors (genetics, metabolism)
• Teratoma (metabolism)