Chrysophanol postconditioning attenuated cerebral ischemia-reperfusion injury induced NLRP3-related pyroptosis in a TRAF6-dependent manner.

Abstract

Individuals who suffer from post-CA (cardiac arrest) brain injury experience higher mortality and more severe functional disability. Neuroinflammation has been identified as a vital factor in cerebral ischemia-reperfusion injury (CIRI) following CA. Pyroptosis induces neuronal death by triggering an excessive inflammatory injury. Chrysophanol possesses robust anti-inflammatory features, and it is protective against CIRI. The purpose of this research was to assess the effect of Chrysophanol postconditioning on CIRI-induced pyroptotic cell death, and to explore its underlying mechanisms. CIRI was induced in rats by CA and subsequent cardiopulmonary resuscitation, and PC12 cells were exposed to oxygen-glucose deprivation/reoxygenation (OGD/R) to imitate CIRI in vitro. It was found that post-CA brain injury led to a notable cerebral damage revealed by histopathological changes and neurological outcomes. The existence of pyroptosis was also confirmed in in vivo and in vitro CIRI models. Moreover, we further confirmed that Chrysophanol, the main bioactive ingredient of Rhubarb, significantly suppressed expressions of pyroptosis-associated proteins, e.g., NLRP3, ASC, cleaved-caspase-1 and N-terminal GSDMD, and inhibited the expression of tumor necrosis factor receptor-associated factor 6 (TRAF6). Furthermore, NLRP3 overexpression neutralized the neuroprotection of Chrysophanol postconditioning, suggesting that pyroptosis was the major neuronal death pathway modulated by Chrysophanol postconditioning in OGD/R. Additionally, the neuroprotection of Chrysophanol postconditioning was also abolished by gain-of-function analyses of TRAF6. Finally, the results demonstrated that Chrysophanol postconditioning suppressed the interaction between TRAF6 and NLRP3. Taken together, our findings revealed that Chrysophanol postconditioning was protective against CIRI by inhibiting NLRP3-related pyroptosis in a TRAF6-dependent manner.

• Authors: Pingping Xia, Murat Marjan, Zhuoyi Liu, Wanqing Zhou, Qian Zhang, Chen Cheng, Minxi Zhao, Yuanyuan Tao, Zhihua Wang, Zhi Ye
• Journal: Experimental neurology (Exp Neurol) Vol. 357 Pg. 114197 (11 2022) ISSN: 1090-2430 [Electronic] United States
• PMID: 35932799 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Anthraquinones
• NLR Family, Pyrin Domain-Containing 3 Protein
• Nlrp3 protein, rat
• TNF Receptor-Associated Factor 6
• Glucose
• chrysophanic acid
• Oxygen

Topics

• Animals
• Anthraquinones
• Brain Injuries
• Brain Ischemia (drug therapy)
• Glucose (metabolism)
• NLR Family, Pyrin Domain-Containing 3 Protein (metabolism)
• Oxygen (pharmacology)
• Pyroptosis
• Rats
• Reperfusion Injury (metabolism)
• TNF Receptor-Associated Factor 6 (metabolism)