Investigation on the cellular mechanism of Prunetin evidenced through next generation sequencing and bioinformatic approaches against gastric cancer.

Abstract

Gastric cancer is the common type of malignancy positioned at second in mortality rate causing burden worldwide with increasing treatment options. More accurate and reliable diagnostic methods/biomarkers are urgently needed. The application of transcriptomics technologies possesses the high efficiency of identifying key metabolic pathways and functional genes in cancer research. In this study, we performed a transcriptome analysis on Prunetin treated AGS cells. A total of 1,118 differentially expressed (DE) genes on Prunetin treated AGS cancer cells, among which 463 were up-regulated and 655 were down-regulated. Notably, around 40 genes were found to be related with necroptosis, among which 16 genes were found to be in close association with Receptor Interacting Protein Kinase (RIPK) family. Validation of the RIPK genes through GEPIA identified 8 genes (NRP1, MNX1, SSRP1, PRDX2, PLRG1, LGALS4, SNX5 and FXYD3) which are highly expressed in stomach cancer were significantly down-regulated in PRU treated samples. In conclusion, the sequencing data explores the expression of RIPK mediated genes through necroptosis signaling network in treating gastric cancer. The futuristic validations on the 8 genes as candidate biomarkers will offer a treatment approach against gastric cancer using PRU.

• Authors: Preethi Vetrivel, Santhi Nachimuthu, Abusaliya Abuyaseer, Pritam Bhagwan Bhosale, Sang Eun Ha, Hun Hwan Kim, Min Young Park, Gon Sup Kim
• Journal: Scientific reports (Sci Rep) Vol. 12 Issue 1 Pg. 11852 (07 13 2022) ISSN: 2045-2322 [Electronic] England
• PMID: 35831348 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022. The Author(s).

Chemical References

• Biomarkers
• DNA-Binding Proteins
• FXYD3 protein, human
• High Mobility Group Proteins
• Homeodomain Proteins
• Intracellular Signaling Peptides and Proteins
• Isoflavones
• MNX1 protein, human
• Membrane Proteins
• Neoplasm Proteins
• Nuclear Proteins
• PLRG1 protein, human
• SSRP1 protein, human
• Transcription Factors
• Transcriptional Elongation Factors
• prunetin

Topics

• Biomarkers
• Computational Biology
• DNA-Binding Proteins (genetics)
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• High Mobility Group Proteins (metabolism)
• High-Throughput Nucleotide Sequencing
• Homeodomain Proteins
• Humans
• Intracellular Signaling Peptides and Proteins
• Isoflavones
• Membrane Proteins (metabolism)
• Neoplasm Proteins (genetics)
• Nuclear Proteins
• Stomach Neoplasms (pathology)
• Transcription Factors
• Transcriptional Elongation Factors (metabolism)