Recent work suggests that
Formoterol could be involved in the metabolic regulation of adipose tissue. It's unknown whether
Formoterol possesses an effect against adipogenesis. Here, we found that
Formoterol prevented adipocyte differentiation by reducing
lipid accumulation, evidenced by reduced
Oil Red O staining, declined intracellular
triglyceride level, and downregulation of adipogenic factors (
PPAR-γ, C/EBPα, and Glut4) in differentiation medium (MDI) stimulated 3T3-L1 preadipocytes. The administration of
Formoterol ameliorated
obesity in high fat diet (HFD) fed mice, which was evidenced by decreased
body weight and ratio of fat/body weight, reduced adipocyte size, and decreased visceral adipocyte tissue weight. Furthermore, the expression level of adipogenic factors in white adipocyte tissues of HFD-fed mice was greatly repressed by
Formoterol. Lastly, thermogenic markers (p-AMPK/AMPK, PGC-1α, and UCP-1) were dramatically upregulated by
Formoterol. Collectively,
Formoterol prevented adipogenesis and
obesity in obese mice by regulating the PPARγ/C/EBPα axis and the AMPK/PGC-1α pathway.