ALK1 controls hepatic vessel formation, angiodiversity, and angiocrine functions in hereditary hemorrhagic telangiectasia of the liver.

Abstract

BACKGROUND AND AIMS:

In hereditary hemorrhagic telangiectasia (HHT), severe liver vascular malformations are associated with mutations in the Activin A Receptor-Like Type 1 ( ACVRL1 ) gene encoding ALK1, the receptor for bone morphogenetic protein (BMP) 9/BMP10, which regulates blood vessel development. Here, we established an HHT mouse model with exclusive liver involvement and adequate life expectancy to investigate ALK1 signaling in liver vessel formation and metabolic function.

APPROACH AND RESULTS:

Liver sinusoidal endothelial cell (LSEC)-selective Cre deleter line, Stab2-iCreF3 , was crossed with Acvrl1 -floxed mice to generate LSEC-specific Acvrl1 -deficient mice ( Alk1HEC-KO ). Alk1HEC-KO mice revealed hepatic vascular malformations and increased posthepatic flow, causing right ventricular volume overload. Transcriptomic analyses demonstrated induction of proangiogenic/tip cell gene sets and arterialization of hepatic vessels at the expense of LSEC and central venous identities. Loss of LSEC angiokines Wnt2 , Wnt9b , and R-spondin-3 ( Rspo3 ) led to disruption of metabolic liver zonation in Alk1HEC-KO mice and in liver specimens of patients with HHT. Furthermore, prion-like protein doppel ( Prnd ) and placental growth factor ( Pgf ) were upregulated in Alk1HEC-KO hepatic endothelial cells, representing candidates driving the organ-specific pathogenesis of HHT. In LSEC in vitro , stimulation or inhibition of ALK1 signaling counter-regulated Inhibitors of DNA binding (ID)1-3, known Alk1 transcriptional targets. Stimulation of ALK1 signaling and inhibition of ID1-3 function confirmed regulation of Wnt2 and Rspo3 by the BMP9/ALK1/ID axis.

CONCLUSIONS:

Hepatic endothelial ALK1 signaling protects from development of vascular malformations preserving organ-specific endothelial differentiation and angiocrine signaling. The long-term surviving Alk1HEC-KO HHT model offers opportunities to develop targeted therapies for this severe disease.

• Authors: Christian David Schmid, Victor Olsavszky, Manuel Reinhart, Vanessa Weyer, Felix A Trogisch, Carsten Sticht, Manuel Winkler, Sina W Kürschner, Johannes Hoffmann, Roxana Ola, Theresa Staniczek, Joerg Heineke, Beate K Straub, Jens Mittler, Kai Schledzewski, Peter Ten Dijke, Karsten Richter, Steven Dooley, Cyrill Géraud, Sergij Goerdt, Philipp-Sebastian Koch
• Journal: Hepatology (Baltimore, Md.) (Hepatology) Vol. 77 Issue 4 Pg. 1211-1227 (04 01 2023) ISSN: 1527-3350 [Electronic] United States
• PMID: 35776660 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.

Chemical References

• Placenta Growth Factor
• Growth Differentiation Factor 2
• Stab2 protein, mouse
• Cell Adhesion Molecules, Neuronal

Topics

• Mice
• Female
• Animals
• Telangiectasia, Hereditary Hemorrhagic (genetics)
• Endothelial Cells (metabolism)
• Placenta Growth Factor (metabolism)
• Liver (pathology)
• Signal Transduction
• Growth Differentiation Factor 2 (metabolism)
• Cell Adhesion Molecules, Neuronal (metabolism)