Development of Biodegradable Osteopromotive Citrate-Based Bone Putty.

Abstract

The burden of bone fractures demands development of effective biomaterial solutions, while additional acute events such as noncompressible bleeding further motivate the search for multi-functional implants to avoid complications including osseous hemorrhage, infection, and nonunion. Bone wax has been widely used in orthopedic bleeding control due to its simplicity of use and conformation to irregular defects; however, its nondegradability results in impaired bone healing, risk of infection, and significant inflammatory responses. Herein, a class of intrinsically fluorescent, osteopromotive citrate-based polymer/hydroxyapatite (HA) composites (BPLP-Ser/HA) as a highly malleable press-fit putty is designed. BPLP-Ser/HA putty displays mechanics replicating early nonmineralized bone (initial moduli from ≈2-500 kPa), hydration induced mechanical strengthening in physiological conditions, tunable degradation rates (over 2 months), low swelling ratios (<10%), clotting and hemostatic sealing potential (resistant to blood pressure for >24 h) and significant adhesion to bone (≈350-550 kPa). Simultaneously, citrate's bioactive properties result in antimicrobial (≈100% and 55% inhibition of S. aureus and E. coli) and osteopromotive effects. Finally, BPLP-Ser/HA putty demonstrates in vivo regeneration in a critical-sized rat calvaria model equivalent to gold standard autograft. BPLP-Ser/HA putty represents a simple, off-the-shelf solution to the combined challenges of acute wound management and subsequent bone regeneration.

• Authors: Xinyu Tan, Ethan Gerhard, Yuqi Wang, Richard T Tran, Hui Xu, Su Yan, Elias B Rizk, April D Armstrong, Yuxiao Zhou, Jing Du, Xiaochun Bai, Jian Yang
• Journal: Small (Weinheim an der Bergstrasse, Germany) (Small) Vol. 18 Issue 36 Pg. e2203003 (09 2022) ISSN: 1613-6829 [Electronic] Germany
• PMID: 35717669 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2022 Wiley-VCH GmbH.

Chemical References

• Bone Substitutes
• Citrates
• Citric Acid
• Durapatite

Topics

• Animals
• Bone Regeneration
• Bone Substitutes
• Bone and Bones
• Citrates
• Citric Acid
• Durapatite
• Escherichia coli
• Rats
• Staphylococcus aureus