Arctigenin inhibits apoptosis, extracellular matrix degradation, and inflammation in human nucleus pulposus cells by up-regulating miR-483-3p.

Abstract

BACKGROUND:

Arctigenin (ATG) is the active ingredient of the Chinese herbal medicine Arctium lappa, with anti-inflammatory and antioxidant effects. Excessive inflammation and cell apoptosis are important causes of intervertebral disc degeneration (IDD). Hence, this study probed into the possible role of ATG in IDD.

METHODS:

Interleukin (IL)-1β (10 ng/ml) was adopted to induce human nucleus pulposus cells (HNPCs) as a cell model for IDD. The effects of different concentrations of ATG (0, 2, 5, 10, 20, 50 μmol/L) on the viability of HNPCs and effects of ATG (10, 50 μmol/L) on the viability of IL-1β-induced HNPCs were detected by cell counting kit-8 (CCK-8). After IL-1β-induced HNPCs were transfected with miR-483-3p inhibitor and/or treated with ATG, cell viability and apoptosis were determined by CCK-8 and flow cytometry; the expressions of miR-483-3p, extracellular matrix (ECM)-related genes, and inflammation-related genes were measured by quantitative real time polymerase chain reaction (qRT-PCR), and expressions of ECM/apoptosis/NF-κB pathway-related proteins were quantified by Western blot.

RESULTS:

ATG had no significant effect on the viability of HNPCs but could promote the viability of IL-1β-induced HNPCs. ATG inhibited apoptosis, ECM degradation, inflammation, and activation of NF-κB pathway in HNPCs induced by IL-1β, but promoted the expression of miR-483-3p. MiR-483-3p inhibitor reversed the above-mentioned regulatory effects of ATG.

CONCLUSION:

Arctigenin suppresses apoptosis, ECM degradation, inflammation, and NF-κB pathway activation in HNPCs by up-regulating miR-483-3p.

• Authors: Zhe Ji, Rui Guo, Zhigang Ma, Hongwei Li
• Journal: Journal of clinical laboratory analysis (J Clin Lab Anal) Vol. 36 Issue 7 Pg. e24508 (Jul 2022) ISSN: 1098-2825 [Electronic] United States
• PMID: 35689566 (Publication Type: Journal Article)
• Copyright: © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.

Chemical References

• Extracellular Matrix Proteins
• Furans
• Lignans
• MIRN483 microRNA, human
• MicroRNAs
• NF-kappa B
• arctigenin

Topics

• Apoptosis (genetics)
• Cells, Cultured
• Extracellular Matrix (metabolism)
• Extracellular Matrix Proteins (genetics)
• Furans (pharmacology)
• Humans
• Inflammation (genetics, metabolism)
• Intervertebral Disc Degeneration (drug therapy, genetics)
• Lignans (pharmacology)
• MicroRNAs (genetics, metabolism)
• NF-kappa B (genetics, metabolism)
• Nucleus Pulposus (metabolism)