Abstract |
In this study, 192 diarrheal fecal samples were collected from 2019 to 2021 for monitoring the molecular prevalence of canine parvovirus 2 (CPV-2) among dogs in Southwest China, and 113 samples were detected as Carnivore protoparvovirus 1-positive. Surprisingly, 28/113 (24.8%) strains were identified as feline parvovirus (FPV)-like viruses based on the key amino acid (aa) residues in VP2. Further, 6 FPV-like strains were successfully isolated and genome sequenced, and phylogenetic trees based on the genome, VP2 and NS1 sequences showed that the 6 FPV-like strains were most genetically related with FPV instead of CPV-2. Interestingly, the VP2 proteins of the FPV-like virus contained all key aa residues typical for FPV and can be 100% identical to that of FPV, but the VP1 intron and NS1 aa sequences exhibited some unique molecular characteristics. The FPV-like isolate could hemagglutinate swine erythrocyte at pH values between 6 and 8, and replicated efficiently in MDCK cell line; moreover, the virus could cause canine systemic infection via oral administration. Further analysis based on VP2 sequences of FPV and CPV-2 in GenBank revealed that the FPV-like virus had already existed among dogs in 4 Asian countries, and have circulated widely in China. This study first confirmed that the FPV-like isolates could efficiently infect dogs, and has been prevalent among dogs in China. Moreover, this study first reported the genome characteristics of the FPV-like virus in dogs, which may represent a novel evolution pattern involving in the cross-species transmission of the virus from cats to dogs.
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Authors | Jiali Wang, Xi Chen, Yuxing Zhou, Hua Yue, Ning Zhou, Hongyi Gong, Cheng Tang |
Journal | Veterinary microbiology
(Vet Microbiol)
Vol. 270
Pg. 109473
(Jul 2022)
ISSN: 1873-2542 [Electronic] Netherlands |
PMID | 35653872
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Topics |
- Animals
- Cat Diseases
(epidemiology)
- Cats
- China
(epidemiology)
- Dog Diseases
(epidemiology)
- Dogs
- Feline Panleukopenia Virus
(genetics)
- Parvoviridae Infections
(epidemiology, veterinary)
- Parvovirus, Canine
- Phylogeny
- Prevalence
- Swine
- Swine Diseases
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