Abstract | BACKGROUND: METHODS: We analyzed the differential expression of the UCHL1 gene in lung adenocarcinoma and normal lung tissues, and the correlation between the UCHL1 gene and prognosis was also analyzed by the bioinformatics database TCGA. Meanwhile, we detected and analyzed the expression of UCHL1 and Ki-67 protein in a tissue microarray (TMA) containing 150 patients with lung adenocarcinoma by immunohistochemistry (IHC) and clinicopathological characteristics by TCGA database. In vitro experiments, we knocked down the UCHL1 gene of A549 cells and detected the changes in cell migration, invasion, and apoptosis. At the same time, we analyzed the effect of UCHL1 on anti- tumor drug sensitivity of lung adenocarcinoma by a bioinformatics database. In terms of the detection rate of lung adenocarcinoma indicators, we analyzed the impact of UCHL1 combined with common clinical indicators on the detection rate of lung adenocarcinoma through a bioinformatics database. RESULTS: In this study, the analysis of UCHL1 protein expression in lung adenocarcinoma proved that obviously higher UCHL1 protein level was discovered in lung adenocarcinoma tissues. The expression of UCHL1 was closely related to poor clinical outcomes. Interestingly, a significantly positive correlation between the expression of UCHL1 and Ki-67-indicated UCHL1 was associated with tumor migration and invasion. Through executing loss of function tests, we affirmed that silencing of UCHL1 expression significantly inhibited migration and invasion of lung adenocarcinoma cells in vitro. Furthermore, lung adenocarcinoma cells with silenced UCHL1 showed a higher probability of apoptosis. In terms of the detection rate of lung adenocarcinoma indicators, we discovered UCHL1 could improve the detection rate of clinical lung adenocarcinoma and affect drug sensitivity. CONCLUSION:
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Authors | Jianbo Yao, Abdusemer Reyimu, Ao Sun, Zaxi Duoji, Wubi Zhou, Song Liang, Suxia Hu, Xiang Wang, Jingjing Dai, Xiaoguang Xu |
Journal | World journal of surgical oncology
(World J Surg Oncol)
Vol. 20
Issue 1
Pg. 153
(May 11 2022)
ISSN: 1477-7819 [Electronic] England |
PMID | 35546675
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Antineoplastic Agents
- Ki-67 Antigen
- UCHL1 protein, human
- Ubiquitin Thiolesterase
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Topics |
- Adenocarcinoma
(drug therapy, genetics)
- Adenocarcinoma of Lung
(drug therapy, genetics)
- Antineoplastic Agents
(therapeutic use)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
- Gene Expression Regulation, Neoplastic
- Humans
- Ki-67 Antigen
(metabolism)
- Lung Neoplasms
(drug therapy, genetics, pathology)
- Oncogenes
- Ubiquitin Thiolesterase
(genetics, metabolism, therapeutic use)
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