DDX17 protects hepatocytes against oleic acid/palmitic acid-induced lipid accumulation.

Abstract

Hepatic lipid accumulation is an initiation factor in fatty liver disease, and promoting a reduction in hepatic lipid accumulation is an important treatment strategy. DEAD box RNA helicase 17 (DDX17) is a member of the DEAD-box family and a molecular chaperone. Previous studies have demonstrated that DDX17 is a transcriptional coregulator of tumorigenesis, inflammation, and macrophage cholesterol efflux. The liver is the main site for lipid metabolism, and metabolic (dysfunction)-associated fatty liver disease (MAFLD) is one of the most common chronic liver diseases. However, the impact of DDX17 on hepatic lipid accumulation has not been verified. In this study, we found, for the first time, that oleic acid/palmitic acid (OA/PA)-induced lipid accumulation was largely abrogated by DDX17 overexpression in both HepG2 (a human hepatocellular carcinoma line) and Hep1-6 (a murine hepatocellular carcinoma line) cells, and this effect was due to a marked reduction in cellular triglyceride (TG) content. Moreover, the overexpression of DDX17 was accompanied by a significant decrease in the expression of genes involved in de novo fatty acid synthesis (FAS, ACC, and SCD-1) in both HepG2 and Hep1-6 cells. In conclusion, DDX17 protected against OA/PA-induced lipid accumulation in hepatocytes through de novo lipogenesis inhibition.

• Authors: Xiaoyi Zhang, Tong An, Xiyue Zhang, Tao Shen, Hongxia Li, Lin Dou, Xiuqing Huang, Yong Man, Weiqing Tang, Jian Li
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 612 Pg. 169-175 (07 05 2022) ISSN: 1090-2104 [Electronic] United States
• PMID: 35533489 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Elsevier Inc. All rights reserved.

Chemical References

• Oleic Acid
• Palmitic Acid
• DDX17 protein, human
• DEAD-box RNA Helicases

Topics

• Animals
• Carcinoma, Hepatocellular (metabolism)
• DEAD-box RNA Helicases (genetics, metabolism)
• Hep G2 Cells
• Hepatocytes (metabolism)
• Humans
• Lipid Metabolism
• Lipogenesis
• Liver (metabolism)
• Liver Neoplasms (genetics, metabolism)
• Mice
• Non-alcoholic Fatty Liver Disease (metabolism)
• Oleic Acid (metabolism, pharmacology)
• Palmitic Acid (metabolism, pharmacology)