Production and metabolic clearance of angiotensinogen in conscious rats as measured by steady-state isotope dilution.
|
| Abstract |
Previous studies on the hormonal regulation of hepatic angiotensinogen relied on in-vitro liver preparations and on the measurement of changes in plasma concentration. In this study 125I-labelled angiotensinogen was used to measure simultaneously the production rate (PR) and metabolic clearance rate (MCR) in conscious rats by the constant-rate infusion and single-injection methods. Male rats received daily s.c. injections of isotonic saline (as control), 1 mg corticosterone acetate (CA), 25 micrograms 17 beta-oestradiol benzoate (OB) or 20 micrograms thyroxine (T4) per 100 g body weight. On day 7 of treatment 125I-labelled angiotensinogen was infused into a jugular vein at a rate of 1 microliter/h by osmotic minipumps and blood samples taken 4, 5 and 6 days later. The PR of angiotensinogen increased from 576 +/- 28 (S.E.M.; n = 9) to 954 +/- 63 (n = 9), 1010 +/- 84 (n = 9) and 2359 +/- 150 (n = 10) micrograms/h per kg following treatment with CA, OB and T4 respectively. In contrast, the PR of rat albumin did not change significantly from 218 +/- 8 (n = 7) mg/h per kg. All three hormones increased MCR from 13 +/- 1 (n = 17) ml/h per kg to 17 +/- 1 (n = 9), 18 +/- 2 (n = 9) and 27 +/- 2 (n = 9) ml/h per kg for CA, OB and T4 respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
|
|---|---|
| Authors | M H Little, C Sernia |
| Journal | The Journal of endocrinology (J Endocrinol) Vol. 112 Issue 3 Pg. 391-7 (Mar 1987) ISSN: 0022-0795 [Print] England |
| PMID | 3549956 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!