Abstract | BACKGROUND & AIMS: METHODS: High-throughput RNA sequencing was used to identify anti-metastatic miRNAs. The relative expression levels of miRNAs were confirmed by qRT-PCR. The biological functions of miRNAs were detected in vitro and in vivo. Circulating tumour cells (CTCs) were enriched from blood samples of HCC patients and cultured by three-dimensional (3D) system. Kaplan-Meier and Cox regression were used to analyse the value of potential target mRNAs on overall survival. RESULTS: miR-2392 was significantly down-regulated in HCC. Overexpression of miR-2392 suppressed proliferation, clonogenicity, mobility, spheroid formation and maintenance of cancer stem cells (CSC)-like characteristics in HCC cells. CTCs from HCC patients with lower serum miR-2392 level had stronger cell spheroid formation ability. A negative correlation between the content of miR-2392 in serum and the number of CTC spheroids had been found. We identified Jagged2 (JAG2) as a direct target of miR-2392. miR-2392 inhibited the expression of JAG2 by targeting 3'-UTR of JAG2. Down-regulation of JAG2 inhibited the overexpression effects of miR-2392 in vitro and in vivo. JAG2 is highly expressed in HCC and is closely related to poor prognosis and survival of patients. CONCLUSIONS: miR-2392 may play a role as a tumour suppressor to guide the individualized precise treatment of HCC.
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Authors | Bin Sun, Weidan Ji, Chunying Liu, Xuejing Lin, Lei Chen, Haihua Qian, Changqing Su |
Journal | Liver international : official journal of the International Association for the Study of the Liver
(Liver Int)
Vol. 42
Issue 7
Pg. 1658-1673
(07 2022)
ISSN: 1478-3231 [Electronic] United States |
PMID | 35485355
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- JAG2 protein, human
- Jagged-2 Protein
- MIRN2392 microRNA, human
- MicroRNAs
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Topics |
- Carcinoma, Hepatocellular
(pathology)
- Cell Line, Tumor
- Cell Proliferation
(genetics)
- Gene Expression Regulation, Neoplastic
- Humans
- Jagged-2 Protein
(genetics, metabolism)
- Liver Neoplasms
(pathology)
- MicroRNAs
(genetics, metabolism)
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