Abstract |
MicroRNA 21 (miRNA-21) is reported to be closely associated with the development of heart failure, cardiac fibrosis and hypertrophy, which could be a promising biomarker for the early-diagnosis of heart failure. Herein, we propose a novel colorimetric miRNA detection method through the integration of duplex-specific nuclease (DSN)-based signal amplification and AuNPs assembly induced colorimetry. Highlights of the proposed method are calculated as: (i) no high temperature hybridization process is required; (ii) DSN enzyme induced recycling provides a favorable detection sensitivity and discrimination of color changes. We believe that the proposed DSN enzyme based colorimetric assay can be applied to heart failure related diagnostics.
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Authors | Zhenfei You, Zeping Yang, Yu Chen, Lei Zhang |
Journal | RSC advances
(RSC Adv)
Vol. 11
Issue 29
Pg. 18068-18073
(May 13 2021)
ISSN: 2046-2069 [Electronic] England |
PMID | 35480196
(Publication Type: Journal Article)
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Copyright | This journal is © The Royal Society of Chemistry. |