Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain.

Abstract

Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).

• Authors: Jenny M Brown, Bao Anh Phan, Nadia Aalling, Gregory J Morton, Michael W Schwartz, Jarrad M Scarlett
• Journal: STAR protocols (STAR Protoc) Vol. 3 Issue 2 Pg. 101329 (06 17 2022) ISSN: 2666-1667 [Electronic] United States
• PMID: 35479117 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't)
• Copyright: © 2022 The Author(s).

Chemical References

• Fibroblast Growth Factor 1
• Glucose

Topics

• Animals
• Diabetes Mellitus, Type 2 (metabolism)
• Fibroblast Growth Factor 1 (pharmacology)
• Glucose (metabolism)
• Hypothalamus (metabolism)
• Mice
• Signal Transduction