Abstract | BACKGROUND: L- theanine, a non- protein amino acid was found principally in the green tea, has been previously shown to exhibit potent anti- obesity property and hepatoprotective effect. Herein, we investigated the effects of L- theanine on alleviating nonalcoholic hepatic steatosis in vitro and in vivo, and explored the underlying molecular mechanism. METHODS: In vitro, HepG2 and AML12 cells were treated with 500 μM oleic acid (OA) or treated with OA accompanied by L- theanine. In vivo, C57BL/6J mice were fed with normal control diet (NCD), high-fat diet (HFD), or HFD along with L- theanine for 16 weeks. The levels of triglycerides (TG), accumulation of lipid droplets and the expression of genes related to hepatocyte lipid metabolic pathways were detected in vitro and in vivo. RESULTS: CONCLUSIONS: Taken together, our findings suggested that L- theanine alleviates nonalcoholic hepatic steatosis by regulating hepatocyte lipid metabolic pathways via the CaMKKβ-AMPK signaling pathway.
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Authors | Juanjuan Liang, Lili Gu, Xianli Liu, Xintong Yan, Xiaowen Bi, Xirui Fan, Jinyi Zhou, Shuai Lu, Lan Luo, Zhimin Yin |
Journal | Nutrition & metabolism
(Nutr Metab (Lond))
Vol. 19
Issue 1
Pg. 29
(Apr 15 2022)
ISSN: 1743-7075 [Print] England |
PMID | 35428314
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |