Polyphyllin I alleviates inflammatory injury in mice with gestational diabetes through AMPK pathway.

Abstract

Our study aimed to detect the effects of polyphyllin I (PPI) on relieving gestational diabetes mellitus (GDM), and the possible mechanism. A mouse model of GDM was constructed. The effects of PPI on GDM mice were evaluated by detecting blood glucose, insulin level, glucose tolerance test, and insulin tolerance test. The inflammation response in GDM and GDM+PPI group were evaluated by enzyme-linked immunosorbent assay (ELISA). The effect of PPI on the offspring of GDM mice was analyzed. In addition, immunoblot assays were performed to investigate the effects of PPI on the AMPK pathway. We found that PPI improved diabetes-related symptoms and decreased serum inflammatory response in GDM mice. In addition, we also found that PPI reduced the tissue damage of GDM mice. We noticed that PPI alleviated inflammatory injury in GDM mice through targeting AMPK pathway. Our findings showed that PPI has the potential to be explored as the drug for GDM treatment.

• Authors: Yaoyao Cai, Fengfeng Xie, Haiou Wang, Qi Zhai, Feng Lin, Shishi Pan
• Journal: General physiology and biophysics (Gen Physiol Biophys) Vol. 41 Issue 2 Pg. 159-164 (Mar 2022) ISSN: 0231-5882 [Print] Slovakia
• PMID: 35416179 (Publication Type: Journal Article)

Chemical References

• Blood Glucose
• Insulin
• polyphyllin I
• AMP-Activated Protein Kinases
• Diosgenin

Topics

• AMP-Activated Protein Kinases (metabolism)
• Animals
• Blood Glucose (metabolism)
• Diabetes, Gestational (drug therapy, metabolism)
• Diosgenin (analogs & derivatives)
• Female
• Insulin (metabolism)
• Mice
• Pregnancy
• Signal Transduction