Abstract |
MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53 -/- mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.
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Authors | Hong Niu, Haichang Li, Ya Guan, Xin Zhou, Zhongguang Li, Serana Li Zhao, Peng Chen, Tao Tan, Hua Zhu, Valerie Bergdall, Xuehong Xu, Jianjie Ma, Jianjun Guan |
Journal | Bioactive materials
(Bioact Mater)
Vol. 18
Pg. 104-115
(Dec 2022)
ISSN: 2452-199X [Electronic] China |
PMID | 35387169
(Publication Type: Journal Article)
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Copyright | © 2022 The Authors. |