Aptamer-Initiated Catalytic Hairpin Assembly Fluorescence Assay for Universal, Sensitive Exosome Detection.

Abstract

Cancer-cell-derived exosomes are regarded as noninvasive biomarkers for early cancer diagnosis because of their critical roles in intercellular communication and molecular exchange. A robust aptamer-initiated catalytic hairpin assembly (AICHA) fluorescence assay is proposed for universal, sensitive detection of cancer-derived exosomes. The AICHA was verified with the specific detection of MCF-7 cell-derived exosomes with a wide calibration range of 8.4 particles/μL to 8.4 × 105 particles/μL and a low detection limit (LOD) of 0.5 particles/μL. The universality of the AICHA method was verified for PANC-1 cell-derived exosomes, the LOD of which was determined to be 0.1 particles/μL. The performances in serum samples were detected with a recovery rate range of 95.45-106.2%, which demonstrates its significant potential for protein biomarker analysis and cancer diagnosis.

• Authors: Jiaqi Zhou, Qiuyuan Lin, Zhipeng Huang, Huiwen Xiong, Bin Yang, Hui Chen, Jilie Kong
• Journal: Analytical chemistry (Anal Chem) Vol. 94 Issue 15 Pg. 5723-5728 (04 19 2022) ISSN: 1520-6882 [Electronic] United States
• PMID: 35377617 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aptamers, Nucleotide

Topics

• Aptamers, Nucleotide (metabolism)
• Biosensing Techniques (methods)
• Exosomes (metabolism)
• Fluorescence
• Humans
• Neoplasms (diagnosis)