MicroRNA hsa-miR-657 promotes retinoblastoma malignancy by inhibiting peroxisome proliferator-activated receptor alpha expression.

Abstract

Retinoblastoma is a familial inherited embryonic neuroretinal malignancy with a low survival rate and poor prognosis. Our study aimed to evaluate the potential interaction between microRNA miR-657 and the peroxisome proliferator-activated receptor alpha (PPARA) in retinoblastoma. Expression of miR-657 and PPARA was analyzed in retinoblastoma tissues and cells using RT-qPCR. Cell proliferation, apoptosis, and migration were measured in retinoblastoma cell lines, and xenografting experiments were performed using nude mice. Our study showed that miR-657 expression was markedly increased, whereas that of PPARA was markedly decreased in retinoblastoma. Additionally, PPARA knockdown enhanced the development of retinoblastoma. miR-657 enhanced the retinoblastoma tumorigenesis by directly inhibiting PPARA expression, suggesting that PPARA targeting by miR-657 facilitates retinoblastoma development by enhancing cell growth. This study provides novel insights into the miR-657- and PPARA-mediated mechanisms underlying retinoblastoma progression and suggests that the interaction between miR-657 and PPARA may serve as an effective target for therapeutic intervention.

• Authors: Xiumei He, Yueyue Feng
• Journal: Anti-cancer drugs (Anticancer Drugs) Vol. 33 Issue 5 Pg. 478-488 (06 01 2022) ISSN: 1473-5741 [Electronic] England
• PMID: 35324527 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.

Chemical References

• MIRN657 microRNA, human
• MicroRNAs
• PPAR alpha
• PPARA protein, human

Topics

• Animals
• Apoptosis (genetics)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation (genetics)
• Gene Expression Regulation, Neoplastic
• Heterografts
• Humans
• Mice
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• PPAR alpha (genetics, metabolism)
• Retinal Neoplasms (genetics)
• Retinoblastoma (genetics, metabolism, pathology)