GSK-3β suppression upregulates Gli1 to alleviate osteogenesis inhibition in titanium nanoparticle-induced osteolysis.

Abstract

Wear particle-induced periprosthetic osteolysis (PPO) have become a major reason of joint arthroplasty failure and secondary surgery following joint arthroplasty and thus pose a severe threat to global public health. Therefore, determining how to effectively suppress particle-induced PPO has become an urgent problem. The pathological mechanism involved in the PPO signaling cascade is still unclear. Recently, the interaction between osteogenic inhibition and wear particles at the implant biological interface, which has received increasing attention, has been revealed as an important factor in pathological process. Additionally, Hedgehog (Hh)-Gli1 is a crucial signaling cascade which was regulated by multiple factors in numerous physiological and pathological process. It was revealed to exert a crucial part during embryonic bone development and metabolism. However, whether Hh-Gli1 is involved in wear particle-induced osteogenic inhibition in PPO remains unknown. Our present study explored the mechanism by which the Hh-Gli1 signaling cascade regulates titanium (Ti) nanoparticle-induced osteolysis. We found that Hh-Gli1 signaling was dramatically downregulated upon Ti particle treatment. Mechanistically, glycogen synthesis kinase 3β (GSK-3β) activation was significantly increased in Ti particle-induced osteogenic inhibition via changes in GSK-3β phosphorylation level and was found to participate in the posttranslational modification and degradation of the key transcription factor Gli1, thus decreasing the accumulation of Gli1 and its translocation from the cytoplasm to the nucleus. Collectively, these findings suggest that the Hh-Gli1 signaling cascade utilizes a GSK3β-mediated mechanism and may serve as a rational new therapeutic target against nanoparticle-induced PPO.

• Authors: Qing Wang, Wei Zhang, Xiaole Peng, Yunxia Tao, Ye Gu, Wenming Li, Xiaolong Liang, Liangliang Wang, Zerui Wu, Tianhao Wang, Haifeng Zhang, Xin Liu, Yaozeng Xu, Yu Liu, Jun Zhou, Dechun Geng
• Journal: Journal of nanobiotechnology (J Nanobiotechnology) Vol. 20 Issue 1 Pg. 148 (Mar 19 2022) ISSN: 1477-3155 [Electronic] England
• PMID: 35305665 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s).

Chemical References

• GLI1 protein, human
• Hedgehog Proteins
• Zinc Finger Protein GLI1
• Titanium
• GSK3B protein, human
• Glycogen Synthase Kinase 3 beta

Topics

• Glycogen Synthase Kinase 3 beta
• Hedgehog Proteins (metabolism)
• Humans
• Nanoparticles
• Osteogenesis
• Osteolysis (chemically induced, drug therapy, metabolism)
• Titanium (pharmacology)
• Zinc Finger Protein GLI1 (pharmacology, therapeutic use)