Abstract | BACKGROUND: METHODS: Several public databases were first used for a preliminary screening of candidate genes. The RNA levels of these candidate genes were then detected in cancer cell lines and the patients serum-derived EVs by PCR Array or digital PCR, respectively. RESULTS: We found that 4 EV-RNAs, ANLN, ITGA6, KRT18, and MMP9, had a lower level in gastrointestinal cancer patients than in benign gastrointestinal diseases patients and healthy controls, while 3 EV-RNAs, ANLN, ITGA6, and KRT18, had a lower level in pancreatic cancer patients than in benign pancreatic diseases patients or healthy individuals. And EV- RNA of MMP9 had a relatively higher level in advanced pancreatic cancer patients than in early-stage patients. Moreover, ROC analysis demonstrated that the determination of the above EV-RNAs could increase the ability of traditional tumor biomarkers to distinguish benign and malignant diseases. CONCLUSIONS:
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Authors | Xuan Zhang, Di Xia, Rui-Xian Wang, Ying-Ting Zhang, Si-Ying Zhang, Cheng Yang, Xiao-Rong Pan, Jian-Hua Tong |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 531
Pg. 36-47
(Jun 01 2022)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 35292253
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers, Tumor
- RNA
- Matrix Metalloproteinase 9
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Topics |
- Biomarkers, Tumor
(genetics, metabolism)
- Extracellular Vesicles
(genetics, metabolism)
- Humans
- Matrix Metalloproteinase 9
(genetics, metabolism)
- Pancreatic Neoplasms
(diagnosis, genetics, metabolism)
- RNA
(metabolism)
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