Comprehensive analysis of negatively correlated miRNA-mRNA regulatory pairs associated with microsatellite instability in colorectal cancer.

Abstract

BACKGROUND:

Several studies have demonstrated that microRNAs (miRNAs) and target mRNAs are associated with different frequencies of microsatellite instability.

OBJECTIVE:

The study aimed to elucidate the profiles of miRNAs and target mRNAs expression and their associations with the phenotypic hallmarks of microsatellite instability in colorectal cancers (CRC) by integrating transcriptomic, immunophenotype, methylation, mutation, and survival data.

METHODS:

Differentially expressed miRNAs (DEmiRNAs) and mRNAs (DEmRNAs) were screened out and then the miRNA-mRNA regulatory pairs were identified through two databases. We verified that the expression levels were detected in 40 microsatellite instable (MSI) and 40 microsatellite stable (MSS) CRC samples and used the logistic regression and the Cox regression method to evaluate the diagnostic and prognostic value of negative regulatory pairs respectively.

RESULTS:

The best diagnostic model that combines miR-31-5p, PLAGL2, miR-361-5p, and RAB27B, which were associated with immune microenvironment, tumor mutation burden (TMB), and overall DNA methylation, could significantly predict microsatellite instability in colon tissues. MiR-31-5p and RAB27B could also predict the overall survival of MSS CRCs.

CONCLUSION:

This study generated a predictive model of the combination of miRNAs and mRNAs to distinguish MSI versus MSS CRCs and elaborated their potential molecular mechanisms and biological functions.

• Authors: Cheng Liu, Xuan Zou, Guoxin Song, Xingchen Fan, Shuang Peng, Shiyu Zhang, Xiangnan Geng, Xin Zhou, Tongshan Wang, Wenfang Cheng, Wei Zhu
• Journal: Cancer biomarkers : section A of Disease markers (Cancer Biomark) Vol. 34 Issue 3 Pg. 471-483 ( 2022) ISSN: 1875-8592 [Electronic] Netherlands
• PMID: 35253734 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• DNA-Binding Proteins
• MicroRNAs
• PLAGL2 protein, human
• RNA, Messenger
• RNA-Binding Proteins
• Transcription Factors

Topics

• Biomarkers, Tumor (genetics)
• Colorectal Neoplasms (pathology)
• DNA-Binding Proteins (genetics)
• Humans
• MicroRNAs (genetics, metabolism)
• Microsatellite Instability
• RNA, Messenger (genetics)
• RNA-Binding Proteins (genetics)
• Transcription Factors (genetics)
• Tumor Microenvironment