Abstract | CONTEXT:
Paeonol (PAE) is the main phytochemical from Cortex Moutan. Its main pharmacological effects are anti-inflammatory and antioxidant, but its cardioprotective effect is unclear. OBJECTIVE: The study investigates the effects and underlying mechanisms of PAE on transverse aortic constriction (TAC)-induced heart failure (HF) in mice. MATERIALS AND METHODS: C57BL/6 mice were randomly divided into five groups: sham, TAC, PAE10 (TAC + PAE 10 mg/kg), PAE20 (TAC + PAE 20 mg/kg) and PAE 50 (TAC + PAE 50 mg/kg). Paeonol was intragastrically administered to mice for 4 weeks. Mice were anaesthetized with pentobarbital sodium and underwent cardiac echocardiography using echocardiography system. Serum levels of atrial natriuretic peptide ( ANP), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). Myocardial apoptosis was detected with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) staining. Haematoxylin- eosin (H&E) and Masson's staining were used for histopathological evaluation. Western and quantitative real-time PCR (qRT-PCR) were performed to detect levels of apoptosis and fibrosis-related proteins. RESULTS: Echocardiography showed PAE improved cardiac function (LVEF: TAC, 52.3±6.8%; PAE20, 65.8±3.6%; PAE50, 71.4±2.5%) and H&E staining showed PAE alleviated myocardial injury (TAC: 1170.3 ± 134.6 μm2; PAE50: 576.0 ± 53.5 μm2). Western and qRT-PCR results showed that PAE down-regulated the levels of ANP, BNP and α-MHC. In addition, TUNEL and western results showed PAE significantly inhibited apoptosis. Masson and western results showed PAE inhibited cardiac hypertrophy. Western results showed the ERK1/2/JNK pathway could be inhibited by PAE. DISCUSSION AND CONCLUSIONS:
Paeonol regulates ERK1/2/JNK to improve cardiac function, which provides theoretical support for the extensive clinical treatment of HF.
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Authors | Xu Chen, Zhiyu Zhang, Xiaowei Zhang, Zhi Jia, Jun Liu, Xinpei Chen, Aiqing Xu, Xue Liang, Guangping Li |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 60
Issue 1
Pg. 562-569
(Dec 2022)
ISSN: 1744-5116 [Electronic] England |
PMID | 35249458
(Publication Type: Journal Article)
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Chemical References |
- Acetophenones
- Cardiotonic Agents
- paeonol
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Topics |
- Acetophenones
(pharmacology)
- Animals
- Aorta
(physiopathology)
- Apoptosis
(drug effects)
- Cardiomegaly
(prevention & control)
- Cardiotonic Agents
(pharmacology)
- Constriction, Pathologic
(complications)
- Disease Models, Animal
- Heart Failure
(drug therapy, physiopathology)
- MAP Kinase Signaling System
(drug effects)
- Male
- Mice
- Mice, Inbred C57BL
- Signal Transduction
(drug effects)
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