Cancer metastasis causes most
cancer-related deaths, and modeling
cancer invasion holds potential in
drug discovery and companion diagnostics. Although 2D cocultures have been developed to study
cancer invasion, it is challenging to recreate the 3D
cancer invasion of an individual
cancer patient. Here, we report an acoustic bioprinting technology that can precisely construct
tumor microtissues for modeling
cancer invasion in 3D. By using acoustic droplet technology, we can precisely encapsulate cancer associated fibroblasts (CAFs) derived from a
colorectal cancer patient into gel droplets and print them into a 3D CAF microtissue. After depositing a
tumor organoid derived from the same patient, our 3D bio-printed microtissue can be used to model
cancer cell migration and invasion from the
tumor organoid to the 3D CAF microtissue. We further used 3D bio-printed microtissues to investigate
cancer invasion dynamics as well as their treatment response using time-lapse imaging. Thus, our acoustic 3D bioprinting technology can be widely used for establishing various microtissues for modeling
cancer invasion and other diseases, highlighting its potential in personalized treatment.