The Coxsackievirus and Adenovirus Receptor Has a Short Half-Life in Epithelial Cells.

Abstract

The coxsackievirus and adenovirus receptor (CAR) is an essential cellular protein that is involved in cell adhesion, cell signaling, and viral infection. The 8-exon encoded isoform (CAREx8) resides at the apical surface of polarized epithelia, where it is accessible as a receptor for adenovirus entering the airway lumen. Given its pivotal role in viral infection, it is a target for antiviral strategies. To understand the regulation of CAREx8 and determine the feasibility of receptor downregulation, the half-life of total and apical localized CAREx8 was determined and correlated with adenovirus transduction. Total and apical CAREx8 has a relatively short half-life of approximately 2 h. The half-life of apical CAREx8 correlates well with adenovirus transduction. These results suggest that antiviral strategies that aim to degrade the primary receptor for apical adenovirus infection will be effective within a relatively short time frame after application.

• Authors: Poornima Kotha Lakshmi Narayan, James M Readler, Mahmoud S Alghamri, Trisha L Brockman, Ran Yan, Priyanka Sharma, Vladislav Snitsarev, Katherine J D A Excoffon, Abimbola O Kolawole
• Journal: Pathogens (Basel, Switzerland) (Pathogens) Vol. 11 Issue 2 (Jan 27 2022) ISSN: 2076-0817 [Print] Switzerland
• PMID: 35215116 (Publication Type: Journal Article)