Abstract | BACKGROUND: METHODS: Saliva samples were collected from healthy volunteers. From these samples, saliva-derived particles were isolated and stained for calcium using calcein or Fluo-4. The salivary particles were also subjected to characterization by flow cytometry and immunoblotting. Internalization of calcein-labeled salivary particles by gingival epithelial cells was visualized by confocal microscopy. RESULTS: We found that calcium-based salivary particles from healthy volunteers varied greatly in size but were enriched in particles of sizes at or greater than 1.5 μm. Immunoblotting analysis of the salivary particles identified several proteins including albumin, fetuin-A, and statherin, which have been found in calcium phosphate particles from other tissues or are known to modulate calcium homeostasis in saliva. In addition, calcium particles were internalized by both gingival epithelial cells and monocyte-derived macrophages. CONCLUSION: Salivary calcium particles were enriched in the micrometer range, internalized by gingival epithelial cells, and contain albumin, fetuin-A and statherin, regulators of particle formation. These characteristics of the calcium-based salivary particles and their biological activities provide a basis for further studies to understand the molecular basis for pathogenesis of periodontitis.
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Authors | Hsin-Hsin Peng, Pei-Rong Huang, John D Young, David M Ojcius |
Journal | Biomedical journal
(Biomed J)
Vol. 44
Issue 6
Pg. 686-693
(12 2021)
ISSN: 2320-2890 [Electronic] United States |
PMID | 35166207
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Chang Gung University. Published by Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Calcium
(analysis, metabolism)
- Epithelium
(metabolism)
- Gingiva
(metabolism, pathology)
- Humans
- Periodontitis
- Saliva
(chemistry)
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