Hepatocellular carcinoma is the second most cause of death among the various
cancers worldwide. Recent research searching an alternative
therapy for
cancer treatment without or less side effects. Many studies indicated the beneficial effects of Enhalus acoroides. There has been no scientific validation on
antioxidant and chemopreventive potential of ethanolic extract E. acoroides against
hepatoma. To assess the hepatoprotective activity of E. acoroides (EEEA) against DEN-induced
hepatoma using Wistar albino rats. Animals were distributed into five groups, each containing six rats. To Group I - control rats -
normal saline given. Groups II, III, IV and V rats were injection of DEN at a dose of 100 mg/kg
body weight i.p. to induce
liver cancer. At the commencement of 6th week, Group III rats supplemented with EEEA at a dose of 200 mg/kg
body weight/day upto 16 weeks. Group IV rats supplemented with EEEA for 1 week before the administration of DEN and continued till the sixteenth week. Group V supplementation of
silymarin at a dose of 100 mg/kg
body weight at the beginning of 6th week after the injection of DEN and continued upto 16 weeks and considered as positive control rats. The efficiency of E. acoroides for its
antioxidant hepatoprotective and activity evaluated in rats against DEN-induced liver damage. The hepatoprotective ability of EEEA at a dose of 200 mg/kg was examined against DEN at a dose of 100 mg/kg/b.w. induced hepatotoxicity and analysed by evaluating serum liver and kidney marker levels,
lipid profile (TG, HDL,
LDL and total
cholesterol) and serum tumour
markers (DNA,
RNA, AFP and CEA). Supplementation of EEEA to DEN treated rats was determined by evaluating various
antioxidant biomarkers (SOD, CAT, GPx, GSH, Vit E and Vit C). Histopathological studies and morphometric gross analysis were also support the consequences of this study. A significant improvement of
antioxidant defence and declined MDA levels within the serum of EEEA treated animals compared to the DEN-induced
hepatoma. The supplementation of EEEA declined the serum liver, kidney and serum tumour marker levels and
lipid profile as comparatively to Group I rats. The histopathological changes were changed on supplementation of EEEA demonstrating its protecting effects on hepatocytes as comparatively to Group I rats. Our significances recognized that
crude extract (
ethanol) of E. acoroides revealed a potential impact against DEN-induced
hepatoma and assists as a superior choice for chemopreventive treatments.