Effects of matcha tea extract on cell viability and peroxisome proliferator-activated receptor γ expression on T47D breast cancer cells.

Abstract

PURPOSE:

In the following work, we investigated the nuclear peroxisome proliferator-activated receptor gamma (PPARγ)-dependent proliferation behavior of breast cancer cells after stimulation with matcha green tea extract (MTE).

METHODS:

T47D cells were stimulated with MTE at concentrations of 5, 10 and 50 µg/ml. Cell viability was assessed using a WST-1 assay after an incubation time of 72 h. PPARγ expression was quantified at the gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level.

RESULTS:

The WST-1 test showed a significant inhibition of viability in T47D cells after 72 h at 5, 10 and 50 µg/ml. The PCR showed an overexpression of PPARγ in T47D cells in all concentrations. At the concentration of 50 µg/ml the expression was significantly increased (p < 0.05). The WB demonstrated a significant quantitative increase of PPARγ at protein level with MTE concentrations of 10 and 50 µg/ml. In addition, there was a negative correlation between the overexpression of PPAR γ and the inhibition of proliferation.

CONCLUSION:

MTE decreases the cell viability of T47D cells and furthermore leads to an overexpression of PPARγ on protein and mRNA level.

• Authors: Simon Keckstein, Constantin Tilgener, Udo Jeschke, Simone Hofmann, Theresa Vilsmaier, Till Kaltofen, Helene Heidegger, Falk Batz, Sven Mahner, Lennard Schröder
• Journal: Archives of gynecology and obstetrics (Arch Gynecol Obstet) Vol. 306 Issue 2 Pg. 451-459 (08 2022) ISSN: 1432-0711 [Electronic] Germany
• PMID: 35079875 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s).

Chemical References

• PPAR gamma
• Plant Extracts
• RNA, Messenger
• Tea

Topics

• Breast Neoplasms (drug therapy)
• Cell Survival
• Female
• Humans
• PPAR gamma (genetics)
• Plant Extracts (pharmacology)
• RNA, Messenger (genetics)
• Tea