Hypervirulent FAdV-4 infection induces activation of the NLRP3 inflammasome in chicken macrophages.

Abstract

Fowl adenovirus serotype 4 (FAdV-4) is the primary causative agent of hepatitis-hydropericardium syndrome (HHS) causing great economic losses to the world poultry industry. The exact factors responsible for the pathogenesis of hypervirulent FAdV-4 have not been completely elucidated. Hypervirulent FAdV-4 infection induces inflammatory damages in accompany with a high level of proinflammatory interleukin-1 beta (IL-1β) secretion in a variety of organs. Investigation of the mechanisms underlying hypervirulent FAdV-4-induced IL-1β secretion would contribute to understanding of the pathogenesis of FAdV-4. Here, we investigated whether FAdV-4 infection activates NLRP3 inflammasome in chicken macrophage cell line HD11. The results showed that stimulation of HD11 with hypervirulent FAdV-4 induced NLRP3- and Caspase-1-dependent secretion of IL-1β. Genetic knockdown of NLRP3 or Caspase-1 expression, a critical component of inflammasome, significantly downregulated IL-1β expression, indicating that activation of the NLRP3 inflammasome contributed to the FAdV-4-induced IL-1β secretion. Moreover, ATP signaling and potassium efflux were involved in the process of NLRP3 inflammasome activation. Our data indicated that hypervirulent FAdV-4 infection induces the activation of NLRP3 inflammasome and followed by massive secretion of IL-1β of macrophages, which thereby contribute to the inflamed lesion of tissues.

• Authors: Baiyu Wang, Huifang Guo, Qilong Qiao, Qing Huang, Panpan Yang, Congcong Song, Mingzhen Song, Zeng Wang, Yongtao Li, Yuhe Miao, Jun Zhao
• Journal: Poultry science (Poult Sci) Vol. 101 Issue 3 Pg. 101695 (Mar 2022) ISSN: 1525-3171 [Electronic] England
• PMID: 35077922 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Inflammasomes
• Interleukin-1beta
• NLR Family, Pyrin Domain-Containing 3 Protein
• Caspase 1

Topics

• Animals
• Caspase 1 (metabolism)
• Chickens (metabolism)
• Inflammasomes (metabolism)
• Interleukin-1beta
• Macrophages (metabolism)
• NLR Family, Pyrin Domain-Containing 3 Protein