It reported that heat generated by near-infrared
laser irradiation of
gold nanorods (AuNRs) effectively inhibited
tumor cells, and the conjugate of
epidermal growth factor receptor monoclonal antibody (EGFRmAb) and
gold nanorods could selectively binded to the surface of
cancer cell membrane expressing EGFR. However, there are few research reports on EGFRmAb-AuNRs in
laryngeal squamous cell carcinoma. Therefore, our study aimed to investigate the photothermal effect of EGFRmAb modified AuNRs in inducing
tumor cell death in an animal model of
laryngeal squamous cell carcinoma. We showed that the conjugates of EGFRmAb and AuNRs selectively entered
laryngeal squamous cell carcinoma cells. We analyzed the parameters of
laser irradiation by controlling the near-infrared to optimize the condition and procedure of targeted treatment in nude mice treated with EGFRmAb and AuNRs. In addition, we examined the safety of the combined
therapy. Test results showed that EGFRmAb-AuNRs inhibited the growth of Hep-2 and CNE-2 cells but not normal epithelial cells, and the semi-inhibitor concentration of EGFRmAb in Hep-2 and CNE-2 cells was 4 pmol/ml and 2 pmol/ml, respectively. AuNRs injected into the
tumor and irradiated by near-infrared
laser effectively inhibited
tumor growth in nude mice without toxic effect in mice. We further confirmed that the apoptosis and
necrosis rates of
tumor cells in mice were highest under 3 W/cm2 near-infrared
laser irradiation and AuNRs minimum concentration of 280 μg/kg. In conclusion, we developed a new method of targeting EGFRmAb combined with AuNRs to achieve photothermal effect in the treatment of
laryngeal squamous cell carcinoma.