Protective effects of Polygonatum kingianum polysaccharides and aqueous extract on uranium-induced toxicity in human kidney (HK-2) cells.

Abstract

The current detoxification options of uranium, a toxic radioactive heavy metal, have obvious side effects. Polygonatum kingianum (PK), a natural product with the function of antioxidant, may be effective in detoxification and prevention of uranium-induced nephrotoxicity. Here, we studied the protective effects of PK polysaccharides (PKP) and aqueous extract (PKAE) on uranium-induced toxicity in human kidney (HK-2) cells. First, the physicochemical properties of PKP and PKAE were characterized. Assays on cultured cells demonstrated that pretreatment with PKP and PKAE significantly increased metabolic activity, relieved morphological impairments, and alleviated apoptosis. The impairments caused by uranium exposure were ameliorated (mitochondrial membrane potential and ATP level increased while reactive oxygen species decreased). Molecular mechanistic studies revealed that PKP and PKAE alleviated uranium-induced cytotoxicity by regulating mitochondria-mediated apoptosis and the GSK-3β/Fyn/Nrf2 pathway. Collectively, our data support the preventive and therapeutic applications of PKP and PKAE for uranium poisoning.

• Authors: Wenjing Li, Libing Yu, Bo Fu, Jian Chu, Chun Chen, Xijian Li, Jiahua Ma, Wei Tang
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 202 Pg. 68-79 (Mar 31 2022) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 35033528 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Polysaccharides
• Reactive Oxygen Species
• Uranium
• Glycogen Synthase Kinase 3 beta

Topics

• Apoptosis
• Glycogen Synthase Kinase 3 beta (metabolism)
• Humans
• Kidney
• Oxidative Stress
• Polygonatum (chemistry)
• Polysaccharides (metabolism, pharmacology)
• Reactive Oxygen Species (metabolism)
• Uranium (toxicity)