miRNA-381-3p Functions as a Tumor Suppressor to Inhibit Gastric Cancer by Targeting Fibroblast Growth Factor Receptor-2.

Abstract

Objectives: MicroRNAs possess essential effects on gastric cancer (GC), whereas the underlying mechanisms have not been fully uncovered. The present work focused on investigating the role of miR-381-3p in GC cellular processes and the possible mechanisms. Materials and Methods: miR-381-3p levels within GC tissues and cells were measured through quantitative real-time polymerase chain reaction (qRT-PCR). This study measured cell proliferation, apoptosis, and metastasis through EdU, colony formation, flow cytometry, and Transwell assays separately. TargetScan was adopted to predict the miR-381-3p targets, whereas luciferase reporter assay was adopted for confirmation. Results: miR-381-3p levels were decreased, whereas fibroblast growth factor receptor-2 (FGFR2) expression was increased in GC. miR-381-3p upregulation inhibited proliferation, migration, and invasion and it promoted the apoptosis of GC cells. Further, FGFR2 overexpression partly reversed the miR-381-3p-mediated impacts on GC cellular processes. Conclusions: This study provides an experimental basis, suggesting the potential of using miR-381-3p as the novel marker for GC. Clinical Trial Registration number: 2020-05.

• Authors: Xiang Gao, Huiqi Liu, Qiong Wu, Rong Wang, Mingyu Huang, Qiang Ma, Yongnian Liu
• Journal: Cancer biotherapy & radiopharmaceuticals (Cancer Biother Radiopharm) Vol. 38 Issue 6 Pg. 396-404 (Aug 2023) ISSN: 1557-8852 [Electronic] United States
• PMID: 35029520 (Publication Type: Journal Article)

Chemical References

• MicroRNAs
• MIRN381 microRNA, human
• Receptors, Fibroblast Growth Factor
• FGFR2 protein, human

Topics

• Humans
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Gene Expression Regulation, Neoplastic
• Genes, Tumor Suppressor
• MicroRNAs (genetics, metabolism)
• Receptors, Fibroblast Growth Factor (metabolism)
• Stomach Neoplasms (pathology)