Abstract |
MicroRNAs ( miRNAs) have emerged as important regulators in the development of cardiovascular diseases. miR-410-3p was shown to play a protective or detrimental role in the progression in cardiovascular events. However, the exact role and the underlying mechanism of miR-410-3p in cardiac hypertrophy have not been documented. The current work was aimed to determine the role and underlying mechanism of miR-410-3p on Angiotensin II (Ang II) induced cardiac hypertrophy. FITC- phalloidin staining was used for determination of cardiomyocyte surface area. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to identify mRNA expression level of hypertrophic markers. Smad7 protein expression level was analyzed using Western blot. Dual- luciferase reporter assay was used to examine the regulatory function of miR-410-3p on Smad7. MiR-410-3p was found significantly up-regulated in Ang II-induced cardiac hypertrophy. MiR-410-3p inhibitor remarkably alleviated cardiomyocyte hypertrophic changes. Dual- luciferase reporter assay result indicated that miR-410-3p directly targeted Smad7 and miR-410-3p inhibitor effectively prevented Ang II triggered down-regulation of Smad7. Moreover, Smad7 overexpression significantly reversed the pro-hypertrophic effect of miR-410-3p. In summary, our findings revealed that miR-410-3p mediated Ang II-induced cardiac hypertrophy via targeting inhibition of Smad7.
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Authors | Guizhi Jia, Chunguang Liang, Wenhui Li, Hongliang Dai |
Journal | Bioengineered
(Bioengineered)
Vol. 13
Issue 1
Pg. 119-127
(01 2022)
ISSN: 2165-5987 [Electronic] United States |
PMID | 34951337
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3' Untranslated Regions
- MIRN410 microRNA, rat
- MicroRNAs
- Smad7 Protein
- Smad7 protein, rat
- Angiotensin II
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Topics |
- 3' Untranslated Regions
- Angiotensin II
(adverse effects)
- Animals
- Cardiomegaly
(chemically induced, genetics, metabolism)
- Cells, Cultured
- Disease Models, Animal
- HEK293 Cells
- Humans
- MicroRNAs
(genetics)
- Myocytes, Cardiac
(cytology, drug effects, metabolism)
- Primary Cell Culture
- Rats
- Smad7 Protein
(genetics, metabolism)
- Up-Regulation
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